Answer critical Thinking question #2 for chapter 6 on page 180. Answers should be submitted in word document with any associated references used
1 E.Coli was incubated with aeration in a nutrient medium containing two carbon sources, and the following growth curve was made from this culture
A) Explain what happened at the time marked x
B) Which substrate provided “better” growth condition for the bacteria? How can you tell?
2 Assume that after washing your hands you leave ten bacterial cells on a new bar of soap. You then decided to do a plate count of a soap after it was left in soap dish for 24 hours. You dilute one gram of the soap 1:106 and plate it on heterotrophic plate count agar. After 24 hours of incubation, there are 168 colonies. How many bacterial were on the soap? How did they get there?
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3) Heat lamps are commonly used to maintain food at about 50 degree C for as long as 12 hours in cafeteria serving lines . The following experiment was conducted to determine whether this practice poses a potential health hazard.
Beef cubes were surface-inoculated with 500,000 bacterial cells and incubated at 43-53degree C to establish temperature limits for bacterial growth. The following results were obtained from heterotrophic plate counts performed on beef cubes at 6 and 12 hours after incubation.
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